Cellulose from Posidonia oceanica Sea Balls (Egagropili) as Substrate to Enhance Streptomyces roseochromogenes Cellulase Biosynthesis

Enhancing Streptomyces cellulase production by supplying lignocellulose biomasses has been poorly investigated so far. In this research the biosynthesis of roseochromogenes ATCC13400 cellulases was increased for first time addition a cellulose fraction (2.5 g·L−1) to growth medium, isolated from marine origin Posidonia oceanica sea balls, generally called egagropili.. shake flasks 4.3 folds, compared control, up 268 U·L−1 in 72 h, with productivity 3.7 U·L−1·h−1, while batch it further enhanced 347 45 h doubled 7.7 U·L−1·h−1 A downstream protocol set coupling two ultrafiltration steps on 10 and 3 kDa membranes recover enzymes supernatant. pool three cellulases, having molecular weights between 115 47 kDa, recovered. The optimal conditions their enzymatic activity were 60 °C pH 5.0, they showed CMCase, FPase β-glucosidase action. conclusion, S. might be considered new cell factory biotechnological that using egagropili, well-known plant waste, as substrate.